Conjugated Primary Antibodies
Chemsis offers a wide variety of conjugated primary antibodies allowing you to analyze multiple targets with ease.
Conjugated Primary Antibodies
Primary antibodies conjugated to fluorophores or chromogenic enzymes are useful tools for direct detection of analytes by a variety of techniques including Western blot, IHC, and flow cytometry.
Conjugated primary antibodies are chemically modified antibodies that allow for the direct attachment of a label, such as a fluorescent dye or enzyme. These conjugated antibodies streamline detection by directly binding to their target, enabling the visualization or detection of specific target molecules in biological samples.
There are two types of detection methods used with antibodies, direct and indirect.
Direct detection uses a primary antibody that is directly conjugated to a label. By conjugating primary antibodies, you can directly detect the target without use of secondary antibodies.
Indirect detection uses a primary antibody that is unconjugated and a conjugated secondary antibody that is specific for the host species of the primary antibody. Indirect detection is the preferred method if the target of interest has a lower level of expression.
Benefits of Using a Primary Conjugated Antibody
Using a conjugated primary antibody for direct detection of analytes eliminates any non-specific binding that may occur with secondary antibodies, thereby reducing background signals.
Species Cross-Reactivity MinimizedUsing a primary conjugated antibody for direct detection of analytes minimizes species cross-reactivity that may occur with secondary antibodies.
Procedures SimplifiedUsing a conjugated primary antibody for direct detection of analytes shortens protocols as only one labeling step is required.
Featured Antibodies for Immune Cell Characterization
| B Cells | Dendritic Cells | Macrophages | Natural Killer Cells | T Cells |
| BCMA | CD1c/BDCA-1 | CD68 | CD56/NCAM-1 | CD3 |
| CD19 | CD11c | CD163 | CD16/Fcy RIII | CD4 |
| CD38 | CD141/BDCA-3 | CD206/MMR | NKp30 | CD8 |
| CD138/Syndecan-1 | SIRP alpha/CD172a | HLA-DR | NKp46 | CD25/IL-2 Ralpha |
| IgM | TNF-alpha | IL-12/IL-35 p35 | IFN-gamma | IL-17/IL-17A |
